Engineering Entrance Exam : GATE : GATE Biochemistry Syllabus 2014

GATE Biochemistry Syllabus 2014

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GATE 2014 Biochemistry Syllabus

Organization of life. Importance of water. Cell structure and organelles. Structure and function of biomolecules : Amino acids, Carbohydrates, Lipids, Proteins and Nucleic acids.

Biochemical separation techniques and characterization: ion exchange, size exclusion and affinity chromatography, electrophoresis, UV – visible, fluorescence and Mass spectrometry.

Protein structure, folding and function : Myoglobin, Hemoglobin, Lysozyme, Ribonuclease A, Carboxypeptidase and Chymotrypsin.

Enzyme kinetics including its regulation and inhibition, Vitamins and Coenzymes.

Metabolism and bioenergetics. Generation and utilization of ATP. Metabolic pathways and their regulation : glycolysis, TCA cycle, pentose phosphate pathway, oxidative phosphorylation, gluconeogenesis, glycogen and fatty acid metabolism.

Metabolism of Nitrogen containing compounds: nitrogen fixation, amino acids and nucleotides. Photosynthesis : the Calvin cycle.

Biological membranes. Transport across membranes. Signal transduction; hormones and neurotransmitters.

DNA replication, transcription and translation. Biochemical regulation of gene expression. Recombinant DNA technology and applications : PCR, site directed mutagenesis and DNA – microarray.

Immune system. Active and passive immunity. Complement system. Antibody structure, function and diversity. Cells of the immune system: T, B and macrophages. T and B cell activation.

Major histocompatibilty complex. T cell receptor. Immunological techniques: Immunodiffusion, immunoelectrophoresis, RIA and ELISA.

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  • ya gate gives good knowledge
    By indu from vellore,tamilnadu on May 30, 2013 at 7:15 pm