GATE Biochemistry Syllabus

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GATE  Biochemistry Syllabus

Section 1

Organization of life; Importance of water; Structure and function of biomolecules : Amino acids, Carbohydrates, Lipids, Proteins and Nucleic acids; Protein structure, folding and function : Myoglobin, Hemoglobin, Lysozyme, Ribonuclease A, Carboxypeptidase and Chymotrypsin.

Section 2

Enzyme kinetics including its regulation and inhibition, Vitamins and Coenzymes ; Metabolism and bioenergetics; Generation and utilization of ATP; Metabolic pathways and their regulation : glycolysis, TCA cycle, pentose phosphate pathway, oxidative phosphorylation, gluconeogenesis, glycogen and fatty acid metabolism; Metabolism of Nitrogen containing compounds: nitrogen fixation, amino acids and nucleotides. Photosynthesis : Calvin cycle.

Section 3

Biochemical separation techniques : ion exchange, size exclusion and affinity chromatography, Characterization of biomolecules by electrophoresis, UV – visible and fluorescence spectroscopy and Mass spectrometry.

Section 4

Cell structure and organelles; Biological membranes; Transport across membranes; Signal transduction; Hormones and neurotransmitters.

Section 5

DNA replication, transcription and translation; Biochemical regulation of gene expression; Recombinant DNA technology and applications : PCR, site directed mutagenesis and DNA – microarray.

Section 6

Immune system : Active and passive immunity; Complement system; Antibody structure, function and diversity; Cells of the immune system : T, B and macrophages; T and B cell activation; Major histocompatibilty complex; T cell receptor; Immunological techniques : Immunodiffusion, immunoelectrophoresis, RIA and ELISA.

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